Cloning and sequencing of mammalian glutathione reductase cDNA

Mammalian Selenoprotein Thioredoxin-glutathione Reductase

Cloning and sequencing of chloroperoxidase cDNA.

An oligod-d(T) 12-18 primed cDNA library has been prepared from Caldariomyces fumago mRNA. A clone containing a full-length insert was sequenced on the supercoiled plasmid, pBR322. The complete primary sequence of chloroperoxidase has been derived. We have also determined about 73% of the peptide sequence by amino acid sequencing. The DNA sequence data matches all of the available known peptide...

Soybean root nodule cDNA encoding glutathione reductase.

GSH is widely distributed in organisms and has diverse functions in protein synthesis, sulfur storage, and protection against a variety of stresses (Meister and Anderson, 1983). GR (EC 1.6.4.2) is a flavoprotein that catalyzes the reduction of GSSG to reduced GSH using NADPH as the reducing cofactor. It is a key enzyme in the GSH-ascorbate cycle, which provides protection against oxidative stre...

Sequencing and expression of a cDNA for human glutathione synthetase.

A human brain cDNA clone encoding glutathione synthetase (EC 6.3.2.3) has been sequenced and expressed in Escherichia coli. The protein is 474 amino acids in length with a subunit molecular mass of 52,352 Da. The recombinant protein exhibits glutathione synthetase activity and occurs as a homodimer. The recombinant glutathione synthetase was purified to homogeneity and had a specific activity o...

Nitrate reductase from squash: cDNA cloning and nitrate regulation.

The assimilation of nitrate in plants involves the reduction of nitrate to ammonia in two steps. The first step requires nitrate reductase, a nitrate-inducible enzyme. When seedlings of squash (Cucurbita maxima L.) were treated with nitrate, both nitrate reductase activity and protein were induced in the cotyledons. Poly(A)(+) RNA was prepared from cotyledons of nitrate-treated seedlings and wa...